Cellular & Molecular Biology ::

The cell culture laboratory is equipped with the required instrumentation for virtually any kind of cell-based experiments. It has two culture rooms with up to P3 level bio-safety containment facilities.

In addition, a separate room is available for media preparation.

All culture rooms are maintained under relatively negative pressure.

Cell Culture Laboratory equipments include :

• Biosafety Cabinets: Maxisafe 2010, Maxisafe 2000 and MSC III (Heto Holten, Denmark) capable of handling up to risk group IV organisms.
• CO2 Incubators (Forma Scientific, USA)
• Inverted Microscopes (Carl Zeiss, Germany)

Mammalian Cell Bioreactor (10Ltr.): Celligen Plus (New Brunswick, USA)

Further, the cell culture area has world class Microscope from Carl Zeiss viz. Axiophot II, with powerful features that allow fluorescence microscopy, digital documentation, image analysis, etc

The Molecular Biology Laboratory is a state-of-the-art setup fully equipped with necessary instruments required to conduct nucleic acid and protein work.

The major instrumentation setup includes :

• DNA & Protein Electrophoresis & Blotting Units
• Gel Documentation System (Bio-Rad)
• PCR Machines (M.J. Research)
• UV Crosslinker and Hybridisation oven (Pharmacia)
• Automated Gel Stainer (Pharmacia)

In addition, other equipments such as refrigerated centrifuges of various capacities (Sigma and Eppendorf, Germany), Vacuum dryer (Eppendorf), UV and visible spectrophotometer are available.

Instrumentation for Drug Discovery :

• SpectraMax Plus, (Molecular Devices, USA) is a Microplate Reader cum Spectrophotometer that can be used for the detection of absorbance at any wavelength in the UV and visible range (190nm - 1000nm).
• Fmax (Molecular Devices, USA) is an advanced Fluorimeter with a high sensitivity and a facility for carrying out kinetic studies.
• Microplate Lumiometer (Applied Biosystems, USA)